| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vega, Isabella M (11)
|
MRWEST |
40.09 |
39.53 |
-0.56 |
| 2 |
Ebanks, Madison (11)
|
MRTRI |
40.00 |
39.69 |
-0.31 |
| 3 |
Ogaki, Chisa (12)
|
MRYMID |
42.27 |
42.01 |
-0.26 |
| 4 |
Vitti, Alexa A (12)
|
MRUN |
43.33 |
42.81 |
-0.52 |
| 5 |
O'Sullivan, Niamh I (12)
|
NJLIFE |
42.79 |
43.69 |
0.90 |
| 6 |
Suzuki, Mako (12)
|
MRYMID |
43.88 |
44.59 |
0.71 |
| 7 |
Ketron, Emily A (11)
|
MREAST |
45.50 |
45.09 |
-0.41 |
| 8 |
Parasnis, Gabriella A (12)
|
MRSS |
45.44 |
45.68 |
0.24 |
| 9 |
Romanov, Victoria B (12)
|
MRPAC |
46.91 |
46.95 |
0.04 |
| 10 |
Hibbert, Laura (11)
|
MRSS |
46.58 |
47.11 |
0.53 |
| 11 |
Mykytenko, Sofia (12)
|
MRTRI$ |
47.83 |
47.13 |
-0.70 |
| 12 |
Gatterdam, Lyla (12)
|
MRSS |
45.62 |
47.28 |
1.66 |
| 13 |
Euyoque, Nicole A (11)
|
MRWEST |
48.79 |
47.66 |
-1.13 |
| 14 |
Chapman, Linnea (12)
|
MRTRI |
50.48 |
48.95 |
-1.53 |
| 15 |
Dooley, Emma C (11)
|
NJLIFE |
46.62 |
49.02 |
2.40 |
| 16 |
Milstein, Jillian E (11)
|
MRPAC |
48.38 |
49.70 |
1.32 |
| 17 |
Khaytin, Julia (11)
|
MRTRI$ |
55.46 |
51.59 |
-3.87 |
| 18 |
Mejia, Jazmine (12)
|
MRYMID |
50.74 |
51.69 |
0.95 |
| 19 |
Sarier-Smith, Hannah F (11)
|
MRPAC |
|
53.39 |
- |
| 20 |
Folowosele, Ayomide (12)
|
NJLIFE |
51.25 |
54.57 |
3.32 |
| 21 |
Ammon, Ayaka (12)
|
MRLIFE |
1:03.27 |
54.63 |
-8.64 |
| 22 |
Iacobazzi, Michela (11)
|
MREAST |
58.00 |
57.87 |
-0.13 |
| 23 |
Sarullo, Isabella (11)
|
NJLIFE |
59.74 |
59.77 |
0.03 |
| 24 |
Milton, Sophia (12)
|
MRTRI |
1:00.61 |
1:00.24 |
-0.37 |
| 25 |
Cruz, Anisa E (11)
|
MREAST |
1:02.00 |
1:01.92 |
-0.08 |
| 26 |
Kortequee, Sia L (12)
|
MRSSL |
1:10.08 |
1:03.93 |
-6.15 |
|
Berezowski, Natalie (12)
|
MRTRI |
1:00.00 |
DQ |
- |